IVUS/VH外周血基因表达得分与斑块体积和表型有关:ATLANTA研究
Original Article

IVUS/VH外周血基因表达得分与斑块体积和表型有关:ATLANTA研究

Parag H. Joshi1, Sarah Rinehart1, Gustavo Vazquez1, Zhen Qian1, Abhinav Sharma1, Hunt Anderson1, Laura Murrieta1, Nancy Flockhart1, Dimitri Karmpaliotis1, Anna Kalynych1, Bela Asztalos2, Michael R. Elashoff3, John Blanchard3, Steven Rosenberg3, Charles Brown1, Szilard Voros4

1Piedmont Heart Institute, Atlanta, GA 30309, USA; 2Boston Heart Diagnostics, Framingham, MA 01702, USA; 3CardioDx, Inc., Palo Alto, CA 94303, USA; 4Stony Brook University Medical Center, Stony Brook, NY. Work was performed while at Piedmont Heart Institute, USA

Corresponding to: Sarah Rinehart, MD, FACC, FSCCT. Piedmont Heart Institute, 1968 Peachtree Road, NW, 95 Building, Atlanta, GA 30309, USA. Email: sarah.rinehart@piedmont.org.

Abstract

背景:一项基于定量RNA测量的综合性外周基因表达得分已被证明可以用于检测冠状动脉狭窄,以避免使用侵入性的X线血管冠状动脉造影。血管内超声及射频反散射(IVUS/VH)已被证实可用于对冠状动脉斑块的体积及其组分进行定量检测,而且可以用于预测患者结果和疗效。然而,IVUS/VH后的外周基因表达与冠状动脉斑块组分之间的关系尚不明确。
方法:分别对进行IVUS/VH的18例患者进行前瞻性外周血基因表达评分(GES)测量。在整个过程中,对所有斑块进行三维定量测量,测量内容包括斑块体积以及斑块组分[ 纤维组织(FI),纤维脂肪组织(FF),坏死核心(NC)和致密钙(DC)]。通过斯皮尔曼等级相关来评估这些数值与GES的关系。
结果:患者平均年龄为(61.1±8.6)岁,67%为男性。IVUS / VH可以展示出1158毫米的冠状动脉解剖成像。1~40为通过验证数据,平均GES为(21.6±9.4)。斯皮尔曼等级相关提示,GES与斑块体积(R2= 0.55,P = 0.018),坏死核心体积(R2= 0.56,P = 0.015),致密钙体积(R2 = 0.60,P = 0.007)以及非钙化斑块体积(R2 = 0.50 P = 0.036)有关。
结论:本初步报告显示,GES升高与大斑块体积、更易损斑块类型(坏死核心和致密钙)有关。综合GES不仅与阻塞性冠状动脉疾病相关,而且也与大斑块体积和更易损表型有关。

关键词: 基因表达,血管内超声,坏死核,斑块体积,易损斑块


Submitted Dec 21, 2012. Accepted for publication Jan 22, 2013.

doi: 10.3978/j.issn.2223-3652.2013.01.02


Cite this article as: Joshi PH, Rinehart S, Vazquez G, Qian Z, Sharma A, Anderson H, Murrieta L, Flockhart N, Karmpaliotis D, Kalynych A, Asztalos B, Elashoff MR, Blanchard J, Rosenberg S, Brown C III, Voros S. A peripheral blood gene expression score is associated with plaque volume and phenotype by intravascular ultrasound with radiofrequency backscatter analysis: results from the ATLANTA study. Cardiovasc Diagn Ther 2013;3(1):5-14. doi: 10.3978/j.issn.2223-3652.2013.01.02