Ultrasound-based stroke/cardiovascular risk stratification using Framingham Risk Score and ASCVD Risk Score based on “Integrated Vascular Age” instead of “Chronological Age”: a multi-ethnic study of Asian Indian, Caucasian, and Japanese cohorts
Original Article on Advanced Imaging in The Diagnosis of Cardiovascular Diseases

Ultrasound-based stroke/cardiovascular risk stratification using Framingham Risk Score and ASCVD Risk Score based on “Integrated Vascular Age” instead of “Chronological Age”: a multi-ethnic study of Asian Indian, Caucasian, and Japanese cohorts

Ankush Jamthikar1, Deep Gupta1, Elisa Cuadrado-Godia2, Anudeep Puvvula3, Narendra N. Khanna4, Luca Saba5, Klaudija Viskovic6, Sophie Mavrogeni7, Monika Turk8, John R. Laird9, Gyan Pareek10, Martin Miner11, Petros P. Sfikakis12, Athanasios Protogerou13, George D. Kitas14, Chithra Shankar15, Andrew Nicolaides16, Vijay Viswanathan17, Aditya Sharma18, Jasjit S. Suri19

1Department of Electronics and Communications Engineering, Visvesvaraya National Institute of Technology, Nagpur, Maharashtra, India;2Department of Neurology, IMIM - Hospital del Mar, Barcelona, Spain;3Annu’s Hospitals for Skin and Diabetes, Nellore, Andra Pradesh, India;4Department of Cardiology, Indraprastha APOLLO Hospitals, New Delhi, India;5Department of Radiology, University of Cagliari, Italy;6Department of Radiology and Ultrasound, University Hospital for Infectious Diseases, Zagreb, Croatia;7Cardiology Clinic, Onassis Cardiac Surgery Center, Athens, Greece;8Department of Neurology, University Medical Centre Maribor, Maribor, Slovenia;9Heart and Vascular Institute, Adventist Health St. Helena, St Helena, CA, USA;10Minimally Invasive Urology Institute, Brown University, Providence, Rhode Island, USA;11Men’s Health Center, Miriam Hospital Providence, Rhode Island, USA;12Rheumatology Unit, National Kapodistrian University of Athens, Athens, Greece;13Department of Cardiovascular Prevention & Research Unit Clinic & Laboratory of Pathophysiology, National and Kapodistrian Univ. of Athens, Athens, Greece;14R & D Academic Affairs, Dudley Group NHS Foundation Trust, Dudley, UK;15Shree Polyclinic and lab, Bangalore, India;16Vascular Screening and Diagnostic Centre and University of Nicosia Medical School, Nicosia, Cyprus;17MV Hospital for Diabetes and Professor M Viswanathan Diabetes Research Centre, Chennai, India;18Division of Cardiovascular Medicine, University of Virginia, Charlottesville, VA, USA;19Stroke Monitoring and Diagnostic Division, AtheroPoint™, Roseville, CA, USA

Contributions: (I) Conception and design: A Jamthikar, JS Suri; (II) Administrative support: D Gupta, JS Suri; (III) Provision of study materials or patients: V Viswanathan, E Cuadrado-Godia, JS Suri; (IV) Collection and assembly of data: V Viswanathan, C Shankar, E Cuadrado-Godia, A Puvvula; (V) Data analysis and interpretation: A Jamthikar, JS Suri; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.

Correspondence to: Dr. Jasjit S. Suri, PhD, MBA, FAIMBE+, FIAUM++. +Fellow American Institute of Medical and Biological Engineering, ++Fellow American Institute of Ultrasound in Medicine, AtheroPoint™, Roseville, Roseville, CA 95661, USA. Email: jasjit.suri@atheropoint.com.

Background: Vascular age (VA) has recently emerged for CVD risk assessment and can either be computed using conventional risk factors (CRF) or by using carotid intima-media thickness (cIMT) derived from carotid ultrasound (CUS). This study investigates a novel method of integrating both CRF and cIMT for estimating VA [so-called integrated VA (IVA)]. Further, the study analyzes and compares CVD/stroke risk using the Framingham Risk Score (FRS)-based risk calculator when adapting IVA against VA.

Methods: The system follows a four-step process: (I) VA using cIMT based using linear-regression (LR) model and its coefficients; (II) VA prediction using ten CRF using a multivariate linear regression (MLR)-based model with gender adjustment; (III) coefficients from the LR-based model and MLR-based model are combined using a linear model to predict the final IVA; (IV) the final step consists of FRS-based risk stratification with IVA as inputs and benchmarked against FRS using conventional method of CA. Area-under-the-curve (AUC) is computed using IVA and benchmarked against CA while taking the response variable as a standardized combination of cIMT and glycated hemoglobin.

Results: The study recruited 648 patients, 202 were Japanese, 314 were Asian Indian, and 132 were Caucasians. Both left and right common carotid arteries (CCA) of all the population were scanned, thus a total of 1,287 ultrasound scans. The 10-year FRS using IVA reported higher AUC (AUC =0.78) compared with 10-year FRS using CA (AUC =0.66) by ~18%.

Conclusions: IVA is an efficient biomarker for risk stratifications for patients in routine practice.

Keywords: Cardiovascular disease (CVD); stroke; risk assessment; carotid intima-media thickness; conventional cardiovascular risk factors (CCVRFs); chronological age (CA); vascular age (VA); integrated vascular age (IVA)

Submitted Dec 20, 2019. Accepted for publication Jan 17, 2020.

doi: 10.21037/cdt.2020.01.16


Mortality and mobility due to cardiovascular diseases (CVD) are the major challenges in global public health (1). Annually, ~17.7 million people experience CVD, out of which 85% die due to heart attack and stroke (1). The prevalence of CVD is even higher in low- or middle-income countries (1). This is due to the lack of resources for the management of CVD (1). In the last decade, the cost for CVD prevention has also boosted to a great extent (2). Especially in the United States, the current annual cost for CVD prevention is around $600 billion which is estimated to reach up to $1.1 trillion in 2035 (2). Thus, in order to prevent the mortalities due to CVD, it is important to look for preventive solutions that can provide the estimate of long-term risk at a reduced cost (3). Furthermore, such preventive tools should also provide an easy understanding of CVD/stroke risk to patients (3,4). This will help them to manage the CVD by controlling the behavioral risk factors such as weight, body mass index (BMI), low-density lipoproteins (LDL) cholesterol, and physical inactivity (4).

At present, conventional cardiovascular risk calculators (CCVRC) models are available that provide an estimate of 10-year CVD/stroke risk to the patients (5-11). Once the risk level is known to the physicians, then using a set of clinical practice guidelines (7,12-16) the strategy and the potential targets can be decided to prevent the onset of CVD events. One common challenge associated with such types of CCVRC is that their absolute risk sometimes underestimates or overestimates the risk profile of the patients (17,18). The reasons for such underestimation or overestimation are (I) ethnicity-specific nature of each risk calculator, (II) varying patients’ demographics or the combination of demographics and blood biomarkers, or (III) dependence on only conventional risk factors (CRF) (17).

Ultrasound imaging has recently shown promising outcomes in atherosclerosis imaging (19-22). There has been a quest to standardize the image-based phenotype measurements from B-mode ultrasound (23). Recently, robust automated tools were developed for quantification of wall thickness and carotid plaque measurements, so-called image phenotypes (24,25) and its morphological characterization (26). Recently, robust methods for wall thickness and carotid plaque measurements were developed using various engineering methods such as signal processing approaches (24), classification-based approaches, boundary-based approaches or fusion of these. These included cIMT (27) and distance measurement methods (28), IMTV (29) and TPA (30) measurements for CCA. This was extended for all segments including bulb (31,32) and further validated using gold standard and computed tomography (33,34) and tested for inter- and intra-observer variability studies (35). Further, we recently demonstrated that these image phenotypes under the automated paradigm can capture both wall thickness and carotid plaque (25). We will use these automated paradigms for our biomarker studies. One solution can be including all the biomarkers of CVD including ultrasound-based imaging phenotypes presented above (19,36) into the risk prediction model (37-40). Another solution can be the development of a tool that provides an easy understating of risk to patients so that they can modify their lifestyle to prevent CVD (41).

One such preventive tool is “Vascular Age (VA)”. Chronological age (CA) is generally converted into an age that is modulated by conventional cardiovascular risk factors (CCVRF) or image-based phenotypes (5,42-46). CRFs such as hyperlipidemia, diabetes mellitus, hypertension, and smoking, all contribute towards the development of atherosclerosis as shown in Figure 1. It shows the changes in the vascular geometry blood vessels, starting from the normal blood vessels at the left side to the completely modified geometry at the right side. This process further results in the aging of the artery. The cross-sectional view can be visualized in Figure 2 which depicts the difference in vascular geometry of the young and the elderly persons. Vascular aging increases the fibrosis that results in increased thickness of the intimal and medial layers (47). Furthermore, the aging of the blood vessels leads to an increase in arterial stiffness (47,48). The VA is an easily understood tool for the patients since it directly relates the cardiovascular risk to the age of blood vessels arteries (42,44). The accurate and easy understanding of the VA leads to an improved lifestyle change of the patients (44). This modifies their routine practice such as consumption of healthier diet, seizing of smoking, reducing/quitting alcohol consumption, and improves physical fitness (44). This was the reason for both Canadian (14) and European guidelines (49) to recommend using the VA measurement during the CVD risk assessment. At present, there is no solid guideline/consensus available that provides the protocols for measuring the VA (43). There exist several models, to compute the VA of a person (5,45,46,50-52). Some of the models are focused on using the CCVRF (5,52) while others use the image-based phenotypes such as carotid intima-media thickness (45,46,50) or total carotid plaque area (53). Although the CCVRF-based VA models are widely adopted for VA computation, such models do not provide the morphological variations in atherosclerotic plaque components. This morphological variation in atherosclerotic plaque tends to change the healthy behaviors in the blood vessels that may lead to an increase in overall VA of a person (4,48). At present, none of the studies have tried to investigate the effect of combining both image-based phenotypes and CCVRF for VA computation for a screening patient. This is the first study that presents an online system for computing integrated vascular age (IVA) by combining both carotid ultrasound image-based phenotype (CUSIP) and CCVRF. The spirit of this design comes from designing online systems by our team (54,55). The concept of integration has been in existence for a while, where the ultimate effect is to improve the performance of the system (56) or improve the prediction of the system. Since the CUS is a non-invasive, economical, and user-friendly imaging modality (57), it was therefore adapted for for developing the IVA. Once the IVA is known, it can then be used to investigate the CVD risk profile of the patients by replacing them with CA in risk prediction models (45,50,51,53,58-60).

Figure 1 The vascular aging process (Courtesy AtheroPointTM, Roseville, CA, USA).
Figure 2 The cross-sectional view of the blood vessels of the (A) young and (B) aged person (Courtesy AtherpPointTM, Roseville, USA).

The objectives of this proposed study are depicted in Figure 3 and are as follows: (I) to investigate the effect of combining both CUSIP and CCVRF on the VA of person, (II) to develop a non-invasive and easy-to-understand tool called IVA, that can provide a true measure of the VA of person, (III) to investigate the improvement in the risk stratification of patients by replacing the CA with the IVA, and (IV) to benchmark the IVA-based risk stratification against the risk stratification perform using CCVRF-driven VA. In this study, a combination of cIMT and glycated hemoglobin was used as a surrogate biomarker for the CVD/stroke events.

Figure 3 Two-stage system for IVA design and performance evaluation. Stage 1: integrated vascular age computation and stage 2: risk stratification and performance evaluation. CCVRF, conventional cardiovascular risk factors; CUSIP, carotid ultrasound image-based phenotypes, IVA, integrated vascular age; EEGS, event-equivalence gold standard; PE, performance evaluation; AUC, area-under-the-curve.


Study population

A multi-ethnic cohort of 648 patients was selected for this retrospective study. Out of 648 patients, 202 were Japanese (Ohashi Medical Center, Toho University, Japan), 314 were Asian-Indian (MV Hospital, Chennai, India), and 132 were Caucasian (Hospital del Mar, Barcelona, Spain). All the patients were approved by the Institutional Review Board. Written consent was obtained from all the study participants. A total of 1,296 CUS scans (648 patients ×2 CUS scans) were collected from both left and right carotid arteries of the patients. Nine CUS scans were excluded from this study due to the poor quality of ultrasound scans showing no visualization of atherosclerotic plaque. Thus, a total of 1,287 CUS scans were used to model the integrated VA and to test the hypothesis of this study. Two expert operators with 15 years of experience in radiology examined all the CUS scans.

Ultrasound image acquisition

Carotid arteries of all the study participants were scanned using a linear array of transducers with a center frequency of 10 MHz (General Electric, India), 7.5 MHz (Aplio XG, Xario, Aplio XV, Toshiba Inc., Tokyo, Japan), and 10 MHz (Sonosite Microma, Spain) respectively, for Asian-Indian, Japanese, and Caucasian patients, respectively. All the patients have analyzed in the supine position with their head tilted backward. The location of the carotid artery was first identified by the transverse scan. After locating the carotid artery, the CUS probe was rotated by 90 degrees to obtain the longitudinal scans of the anterior and posterior walls of the CCA. A measurement guideline recommended by the American Society of Echocardiography was followed during the measurement. A detailed protocol for image acquisition was discussed in our previous publications (24,37,40,61).

Development of IVA

Figure 4 shows the IVA development architecture of the proposed system. At first, CUSIP and the CRF from three different databases (Japan, Asian Indian, and Italy) were combined to form a complete dataset of 1,287 samples. Then, the system architecture was divided into two parts: (I) training paradigm and (II) testing paradigm. K-fold data-partitioning protocol (K =5, 10, N-1, where N is the sample size) was followed to obtain the training and testing dataset. The details of the K-fold data-partitioning have been given in our previous publications (62,63). In the training paradigm, the average cIMT (cIMTave) and CRF were labeled as “training CUSIP” and “training CCVRF”. Both the training CUSIP” and “training CCVRF” were then separated into three risk categories (low-risk, moderate-risk, and high-risk) based on the average cIMT (cIMTave) threshold points (lower threshold of 0.5 mm and an upper threshold of 0.9 mm). These risk-category dependent CUSIP and CCVRF were then used as input covariates for the linear regression and the multivariate linear regression (MLR) models, respectively. In both the linear regression and MLR models, the CA was used as the dependent variable.

Figure 4 The internal architecture of the proposed system. CUSIP, carotid ultrasound image-based phenotypes; CCVRF, conventional cardiovascular risk factors; MLR, multivariate linear regression; VA, vascular age; CVD, cardiovascular diseases.

Since the age in both the statistical models was determined by the CUSIP and the CCVRF, it was termed as ‘CUSIP-driven vascular age’ and ‘CCVRF-driven vascular age’, respectively. The corresponding coefficients of the linear regression-based CUSIP-driven VA model and the MLR-based CCVRF-driven VA model were then combined using a linear combination, which when combined can be used to transform the test patient to predict an IVA of a test patient. Note that the IVA considers the effect of both the CCVRF and the cIMT. The IVA was then replaced with CA in the FRS-based calculator to risk stratify the patients. A combination of cIMT and HbA1c was used as a response variable or event equivalence gold standard to perform the risk stratification. This study hypothesized that the FRS with IVA as input covariate (FRSIVA) can provide a better CVD risk stratification compared to the FRS with CA as input covariate (FRSCA). From here on, when we call FRSIVA, means FRS is computed using IVA. Similarly, FRSCA, means FRS is computed using CA. Such a combinational approach of using two different types of risk factors as discussed in our previous studies (37,63,64). The performance of FRSIVA was evaluated against the FRSCA based on the area-under-the-curve (AUC) analysis.

Validation of risk stratification using atherosclerosis CVD (ASCVD) calculator

In order to validate the performance of IVA against the CA, CVD risk stratification was performed using the ASCVD risk calculator, which was proposed by the American College of Cardiology (ACC) and the American Heart Association (AHA) (7). The CVD risk stratification was performed (I) by computing the 10-year risk using ASCVD calculator with IVA, instead of CA, as input covariate and (II) stratifying the patients into low- or high-risk category using a predefined risk threshold of 7.5% (7). The performance of ASCVDIVA was also evaluated against the ASCVDCA based on the AUC analysis. At last, to validate the hypothesis, we showed that the risk stratification performed using the FRS calculator (5) and ASCVD calculator (7) should provide a higher AUC with IVA, instead of CA as input covariate.

Statistical analysis

All the baseline characteristics presented in Table 1 are expressed as mean ± standard deviation for continuous variables and as a percentage for categorical variables. The statistical significance was tested using a two-tailed students’ t-test for continuous variables and chi-square tests for categorical variables. A statistical significance was determined for P value <0.05. All the statistical analysis was performed using SPSS23.0 and MATLAB17b. Receiver operating characteristics (ROC) was used to compare FRSIVA and FRSCA. An FRS threshold of 20% was selected to risk-stratify the patients into either low-risk (FRS <20%) or high-risk (FRS ≥20%) category. An AUC was used as a primary performance evaluation metric for this proposed study. A combination of cIMT and HbA1c was used as a response variable to perform the ROC analysis. This response variable also serves the purpose of combinational event equivalence gold-standard to risk stratify the patients. A similar type of combinational approach of deriving a response variable using two risk factors was proposed the Cuadrado-Godia et al. (64) and Khanna et al. (37). At baseline, patients were classified into the high-risk category if cIMT ≥0.9 mm and HbA1c ≥6.5%.

Table 1
Table 1 Baseline characteristics for the study participants
Full table

Power analysis and sample size

In general, data samples should not be identical. In this proposed study, a standardized protocol was followed that selects the data samples at different locations of the same human body (23,65,66). Although the left and right carotid arteries have similar genetic formation and functionality, they work independently along two different pathways (19,40,67). Furthermore, the deposition of atherosclerotic plaque within the blood vessel is completely random and multifocal. Thus, a CUS scan from the left and right carotid artery was collected, yielding a total sample size of 1,287. The validity of the selected sample size of 1,287 CUS scans was tested using a power analysis. Power analysis suggests the smallest sample size required to perform the risk stratification of patients with higher predictive power. In this study, the population refers to the multicenter cohort of Japan, South-India, and Spain. A confidence level of 95% was selected for this study with a margin of error (MoE) of 5% and a data proportion () of 0.5. The desired sample size (n) for the true population was computed using, n=[(Z*)2×(P^(1-P^)ΜOΕ2)] (68). The resultant sample size with a 95% confidence level and 5% of MoE was ~384. Thus recruited sample size (1,287) was ~235% higher compared to the desired sample size of 384. This indicates that the selected sample size was sufficient to perform the statistical analysis.


Baseline characteristics

Table 1 indicates the baseline characteristics of the study participants. A cohort of 648 patients (486 males and 162 females) was analyzed in this study. In a pool of 648 patients, average CA was 56.83±13.86 years (ranging between 24 and 88 years), HbA1c was 7.26%±2.06% (ranging between 4.80% and 14.30%), LDL cholesterol was 103.13±37.08 mg/dL (ranging between 26 and 259 mg/dL), HDL cholesterol was 42.81±13.57 mg/dL (ranging between 19 and 119 mg/dL), total cholesterol was 174.98±49.92 mg/dL (ranging between 60 and 435 mg/dL), SBP was 128.44±13.26 mmHg (ranging between 90 and 182 mmHg), DBP was 80.13±8.59 mmHg (ranging between 90 and 182 mmHg), and triglyceride was 159.49 mg/dL. Out total patients, 232 (35.80%) patients were hypertensive, 351 patients were diabetic, and 140 (21.60%) patients were smokers. The criteria for hypertension were SBP ≥130 or DBP ≥80 mmHg or treatment with hypertensive medication. Similarly, the criteria for diabetes were HbA1c ≥6.5%. Table 1 also shows the baseline characteristics of patients based on two risk categories: low- and high-risk.

CA vs. VA for different risk categories and different partition protocols (K)

Figure 5A,B,C,D shows the comparison between (I) average values of IVA, (II) three types of VA (FRS-based, SCORE chart-based, and cIMTave-based), and (III) CA using four types of data-partitioning protocols (5-, 10-fold, Jack-Knife, and TT). The comparison between all the four types of VA techniques and the CA was made using the three risk classes (low-, moderate-, and high-risk). The risk classes were determined using cIMTave values. From the Figure 5A,B,C,D it was found that mean IVA of all the samples was (I) comparable to mean CA in low-risk category (mean CA =41 years, mean IVA =41 years), (II) slightly higher/comparable than the mean CA in moderate risk category (mean CA =55 years, mean IVA =57 years), and (III) significantly higher compared to mean CA of the high-risk category (mean CA =68 years, mean IVA =92 years). A similar trend of increment in VA was also observed in the other three types of VA techniques (FRS-based, SCORE chart-based, and cIMTave-based). Table S1 in the Appendix compares the average IVA (row R5) against (I) the three types average VA (row R2: FRS-based, row R3: SCORE chart-based, and row R4: cIMTave-based) and the (II) average CA (row R1: CA). This comparison was made based on the three different risk categories (column C1 to column C3) which were derived using cIMTave threshold points (lower threshold of 0.5 mm and an upper threshold of 0.9 mm). This identification of risk categories has already been discussed in the “Development of Integrated Vascular Age” section.

Figure 5 Comparison between the average values of IVA, the types of the average values of VA [FRS-based (5), SCORE chart-based (52), and cIMTave-based (46)], and chronological age for four types of data-partitioning protocols. (A) 5-fold data-partitioning, (B) 10-fold data-partitioning, (C) Jack-Knife data-partitioning, and (D) TT data-partitioning. IVA, integrated vascular age; VA, vascular age; FRS, framingham risk score.
Table S1
Table S1 Risk category-based comparison between average IVA against (I) the three types of average VA [FRS-based (5), SCORE chart-based (52), and cIMTave-based (46)] and (II) average chronological age
Full table

FRS using Integrated Vascular Age (FRSIVA) vs. Chronological Age (FRSCA)

In order to evaluate the performance of the proposed IVA, the 10-year risk of CVD was computed using FRS (5) in two different scenarios: (I) considering IVA as input covariate (FRSIVA) and (II) by considering the CA as input covariate (FRSCA). FRS computed using both of these scenarios were used to risk-stratify the patients into two risk categories (low- or high-risk) using the response variable. Figure 6A shows the ROC curve that evaluates the performance of FRSIVA [solid black curve in Figure 6A] as against the FRSCA (solid red curve in Figure 6A). FRSIVA reported the higher AUC (AUC =0.78, P<0.0001) compared to FRSCA (AUC =0.66, P<0.0001) by ~18%. This has clearly indicated the higher risk prediction ability of the IVA when used in FRS instead of the CA. A similar trend was also observed for four types of data-partitioning protocols as indicated by Figure S1 of the Appendix.

Figure 6 Receiver operating characteristics curve indicating the performance of integrated vascular age against the chronological age and the three types of VA computations methods. (A) FRS computed using IVA against the FRS computed using chronological age, VA computed using FRS (5), VA computed using SCORE chart (52), and VA computed using cIMT-based model developed by Linda model (46); (B) ASCVD computed using IVA against the ASCVD computed using chronological age, VA computed using FRS, VA computed using SCORE chart, and VA computed using Linda model. FRS, framingham risk score; CA, chronological age; VA, vascular age; ASCVD, atherosclerosis CVD; IVA, integrated vascular age.
Figure S1 Comparison of performance of risk stratification using IVA, risk stratification using three types of VA [FRS-based (5), SCORE chart-based (52), and cIMTave-based (46)], and risk stratification using chronological age for four types of data-partitioning protocols. (A) 5-fold data-partitioning, (B) 10-fold data-partitioning, (C) Jack-Knife data-partitioning, and (D) TT data-partitioning. FRS, framingham risk score; CA, chronological age; VA, vascular age; IVA, integrated vascular age; AUC, area-under-the-curve; TT, time-triggered.

FRS using IVA vs. FRS using three types of VA techniques (VAcIMT, VAFRS, and VAscore)

Figure 6 also compares the performance of IVA against the three different types of VA: (I) VA computed using Linda’s model (VAcIMT) (46), (II) VA computed using FRS calculator (5) (VAFRS), and (III) VA computed using SCORE chart (52) (VAscore). Among all these types of techniques, VA computed using the cIMT-based model developed by Linda’s (46) was solely based on cIMT values whereas the VA computed using the FRS calculator (5) and SCORE chart (52) used the CRFs. The AUC values for the 10-year risk computed using FRS calculator (Figure 6A, Table 2) with all three types of vascular ages are: (I) VA using Linda’s model (AUC =0.90, P<0.0001), (II) VA using FRS (AUC =0.62, P<0.0001), and (III) VA using SCORE chart (AUC =0.62, P<0.0001). From Figure 6A, it should be noted that the AUC values of FRSIVA (black solid line) and FRSVA(cIMT) (pink dotted line) were higher compared to FRSVA(SCORE) (blue dotted line) and FRSVA(FRS) (black dotted line). Furthermore, the AUC value for FRSIVA (AUC =0.78, P<0.0001) was higher than the AUC values of both the CCVRF-based FRSVA(SCORE) and FRSVA(FRS) by ~26%. However, the AUC value for FRSIVA (black solid line) was found to be comparable with the AUC value for FRSVA(cIMT) (pink dotted line). The reason for the lower AUC value for the IVA model was due to the proprietary dataset used by Linda et al. (46), which only contained low-to-moderate risk patients (cIMTave ~0.6 mm). Thus, such linear regression coefficients may not be suitable for patients with high baseline risk (for example in the proposed study) which could further provide a bias during risk stratification. This was the reason for a difference in AUC values between Linda’s model and all the other VA models including IVA.

Table 2
Table 2 Comparing the performance of IVA-based risk stratification using FRS against chronological age and three types of VA technique
Full table

Validation and consistency analysis against ASCVD calculator

The atherosclerosis CVD (ASCVD) risk calculator was developed by the American College of Cardiology/American Heart Association and is widely used for 10-year CVD risk estimation (7). In this proposed study, the performance of the IVA was also tested by replacing with CA in the ASCVD calculator (Figure 6B and Table S2). The ASCVD computed using IVA (ASCVDIVA) reported the higher AUC (AUC =0.72, P<0.0001) compared to the ASCVD computed using CA (ASCVDCA) (AUC =0.65, P<0.0001) by ~9%. This further provided validation of our results obtained using FRSIVA. Furthermore, when the risk stratification based on ASCVDIVA was compared against the risk stratification using three types of VA [FRS-based VA (5), SCORE chart-based (52), cIMTave-based VA by Linda et al. (46)], the AUC values showed consistent results as observed using FRS calculator (Figure 6B).

Table S2
Table S2 Risk stratification metrics for 10-year risk computed using IVA against the 10-year risk computed using (I) chronological age and (II) 3 types of traditional vascular ages
Full table


In this study, a novel IVA was proposed that combines the effect of both CRFs and the CUSIP such as cIMTave. The IVA was then used as input covariate to FRS for risk stratification. We showed that FRSIVA (AUC =0.78, P<0.0001) performed better compared against FRSCA, (AUC =0.66, P<0.0001) by 18%. The risk-stratification results with IVA were also validated using the ASCVD calculator. The risk stratification using ASCVDIVA reported higher AUC (AUC =0.72, P<0.0001) compared to the ASCVDVA (AUC =0.65, P<0.0001) by ~11%. This clearly indicates that the IVA can be replaced with CA while performing the long-term cardiovascular risk assessment. Furthermore, while performing the risk CVD risk stratification, this study showed a higher predictive power of integrated risk factors (CUSIP + CRF) compared to CCVRF or CRF alone.


The proposed IVA was benchmarked against 11 studies (row R1 to row R11) that were presented in Table 3. Eight out of 11 studies (column C3, row R1-R3, R5-R6, R8-R9, R11) used the CUSIPs such as cIMT or TPA for vascular age measurement. The studies presented D’Agostino et al. (5) (row R4) and Cuende et al. (52) (row R7) used the CCVRF to measure the vascular age of patients. All the studies that used CUSIP for VA measurement have used the linear regression-based percentile models (or nomograms) to compute the VA. Whereas the CCVRF-based vascular age measurement uses the proportional hazard models such as cox-regression [used by FRS-based point model (5)] or Weibull regression models [used by SCORE chart et al. (52,70)]. Except for the proposed study (row R12), none of the previously published studies have tried to combine the effect of both CCVRF and the CUSIP for VA measurement (column C4). It should also be noted that the proposed IVA calculator was modeled using the three different types of ethnicities (row R12 and column C2). In order to test the performance of vascular age for risk stratification of patients, most of the studies (column C10) used the FRS as a risk calculator. In our proposed study we also tested the performance of IVA using FRS which was further validated using the ASCVD calculator. The ASCVD is the recently developed CCVRC by the American College of Cardiology/American Heart Association (ACC/AHA) which is widely used for CVD risk assessment.

Table 3
Table 3 Studies that used the concept of vascular age for CVD risk assessment
Full table

Comparison of complete MLR model vs. partial MLR model

The proposed model of IVA measurement comprised of both the linear regression (for cIMT-based vascular age) and MLR (for CCVRF-based vascular age). This was also referred to as a partial MLR-based model. On the contrary, we also tested a complete MLR model with both CCVRF and cIMT as input covariates. The CA of a person was used as a dependent variable for the MLR analysis. Such completely MLR-based IVA when added to the FRS by replacing the CA, resulted in an improvement in the AUC by ~5% (AUC of FRS using IVA =0.74 and AUC of FRS using CA =0.70). Figure 7 shows the ROC curve for FRS using CA and VA as an input argument. Although there is an improvement in AUC by 5%, it is less compared to the partial MLR-based IVA proposed in this study resulted in improvement in AUC by ~18%. Thus a partial MLR-based model opted for this study.

Figure 7 Receiver operating characteristics curve for the FRS calculator using (I) CA as input argument (blue curve) and (II) complete MLR-based integrated VA as input argument (red curve). FRS, framingham risk score; CA, chronological age; IVA, integrated vascular age; AUC, area-under-the-curve.

Strengths, limitations, and future scope

The proposed study had following strengths: (I) this was the first study which combined the effect of CCVRF and cIMT to measure the vascular age of person, (II) this was the first attempt to combined three different types of ethnicities (Japanese, Asian-Indian, and Caucasian) to measure the vascular age, and (III) the propose integrated calculator was validated with two types 10-year CCVRC such FRS and ASCVD.

Although the power analysis validated the selected sample size, we intend to extend the analysis to a longitudinal trial with a large diversified cohort of varying ethnicities. Furthermore, it should be noted that the 10-year risk stratification using the proposed IVA provides a trade-off performance between the cIMT-based vascular and CCVRF-based vascular age. This is because of the (I) higher AUC value for the 10-year risk stratification using cIMT-based vascular age compared to the proposed IVA and (II) lower AUC value for the 10-year risk stratification using CCVRF-based vascular age compared to the IVA.

The proposed study development of IVA can further be extended to an automated machine learning-based risk assessment system (3,71) where IVA can be used as an important input feature. More features such as grayscale information from the images can be added (55,72,73). Another extension can be accomplished where CUSIP can be used from deep learning paradigms (74-77).


The proposed study reported the higher potential of integrated vascular compared to (I) CA and (II) to the CRF-based vascular age. Thus, replacing the “chronological age” with the “integrated vascular age” could provide a better CVD risk assessment. CUSIPs such as cIMT can provide a better understanding of the atherosclerosis plaque build-up in the blood vessels. They reflect the overall health of arteries and can provide information about the true arterial age of a person. This was the primary reason for boosting the AUC when cIMT was integrated with the CCVRFs in the vascular age model.


AtheroEdge™ and AtheroEdge Composite Risk ScoreAtheroEdge Composite Risk ScoreTM (38) are trademarks of AtheroPoint™, California, USA. All data were collected during the part of the collaboration between AtheroPoint™ and AtheroPoint™’s clinical partners. All intellectual property used in this manuscript is owned by AtheroPoint™ and protected by US patents; Ankush Jamthikar, MTech would like to thank the Ministry of Human Resource and Development, Government of India to provide financial support for his PhD studies at Visvesvaraya National Institute of Technology, Nagpur, India.

Funding: None.


Provenance and Peer Review: This article was commissioned by the Guest Editor (Luca Saba) for the series “Advanced Imaging in The Diagnosis of Cardiovascular Diseases” published in Cardiovascular Diagnosis and Therapy. The article was sent for external peer review organized by the Guest Editor and the editorial office.

Conflicts of Interest: The series “Advanced Imaging in The Diagnosis of Cardiovascular Diseases” was commissioned by the editorial office without any funding or sponsorship. LS served as the unpaid Guest Editor of the series and serves as an unpaid editorial board member of Cardiovascular Diagnosis and Therapy from July 2019 to June 2021. JS is affiliated to AtheroPointTM, focused in the area of stroke and cardiovascular imaging. The authors have no other conflicts of interest to declare.

Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). All the patients were approved by the Institutional Review Board. Written consent was obtained from all the study participants.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.


  1. Organization WH. Cardiovascular diseases (CVDs): Key facts by WHO May 2016. 2017. Available online: http://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)
  2. Association AH. Cardiovascular disease: A costly burden for America projections through 2035. 2017. Available online: https://healthmetrics.heart.org/cardiovascular-disease-a-costly-burden/
  3. Jamthikar A, Gupta D, Khanna NN, et al. A low-cost machine learning-based cardiovascular/stroke risk assessment system: integration of conventional factors with image phenotypes. Cardiovascular diagnosis and therapy 2019;9:420-30. [Crossref] [PubMed]
  4. Nilsson P. Vascular age: how can it be determined? What are its clinical applications? Medicographia 2015;37:454-60.
  5. D’agostino RB, Vasan RS, Pencina MJ, et al. General cardiovascular risk profile for use in primary care: the Framingham Heart Study. Circulation 2008;117:743-53. [Crossref] [PubMed]
  6. Hippisley-Cox J, Coupland C, Brindle P. Development and validation of QRISK3 risk prediction algorithms to estimate future risk of cardiovascular disease: prospective cohort study. BMJ 2017;357:1-12. [PubMed]
  7. Goff DC, Lloyd-Jones DM, Bennett G, et al. 2013 ACC/AHA guideline on the assessment of cardiovascular risk: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. J Am Coll Cardiol 2014;63:2935-59. [Crossref] [PubMed]
  8. Ridker PM, Buring JE, Rifai N, et al. Development and validation of improved algorithms for the assessment of global cardiovascular risk in women: the Reynolds Risk Score. JAMA 2007;297:611-9. [Crossref] [PubMed]
  9. Organization WH. WHO/ISH cardiovascular risk prediction charts. Prevention of cardiovascular disease: guideline for assessment and management of cardiovascular risk [cited 2011 May 12] Geneva: WHO 2007.
  10. Stevens RJ, Kothari V, Adler AI, et al. The UKPDS risk engine: a model for the risk of coronary heart disease in Type II diabetes (UKPDS 56). Clin Sci (Lond) 2001;101:671-9. [Crossref] [PubMed]
  11. Kothari V, Stevens RJ, Adler AI, et al. UKPDS 60: risk of stroke in type 2 diabetes estimated by the UK Prospective Diabetes Study risk engine. Stroke 2002;33:1776-81. [Crossref] [PubMed]
  12. Perk J, De Backer G, Gohlke H, et al. European Guidelines on cardiovascular disease prevention in clinical practice (version 2012). The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of nine societies and by invited experts). Eur Heart J 2012;33:1635-701. [Crossref] [PubMed]
  13. Prevention EAfC. ESC Committee for Practice Guidelines (CPG) 2008-2010 and 2010-2012 Committees, Rehabilitation, Reiner Z, et al. ESC/EAS Guidelines for the management of dyslipidaemias: the Task Force for the management of dyslipidaemias of the European Society of Cardiology (ESC) and the European Atherosclerosis Society (EAS). Eur Heart J 2011;32:1769-818. [PubMed]
  14. Anderson TJ, Grégoire J, Hegele RA, et al. 2012 update of the Canadian Cardiovascular Society guidelines for the diagnosis and treatment of dyslipidemia for the prevention of cardiovascular disease in the adult. Can J Cardiol 2013;29:151-67. [Crossref] [PubMed]
  15. Anderson TJ, Grégoire J, Pearson GJ, et al. 2016 Canadian Cardiovascular Society Guidelines for the Management of Dyslipidemia for the Prevention of Cardiovascular Disease in the Adult. Can J Cardiol 2016;32:1263-82. [Crossref] [PubMed]
  16. Duerden M, O’Flynn N, Qureshi N. Cardiovascular risk assessment and lipid modification: NICE guideline. Br J Gen Pract 2015;65:378-80. [Crossref] [PubMed]
  17. Perk J, De Backer G, Gohlke H, et al. European Guidelines on cardiovascular disease prevention in clinical practice (version 2012). The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of nine societies and by invited experts). Eur Heart J 2012;33:1635-701. [Crossref] [PubMed]
  18. Allan GM, Nouri F, Korownyk C, et al. Agreement among cardiovascular disease risk calculators. Circulation 2013;127:1948-56. [Crossref] [PubMed]
  19. Suri JS, Kathuria C, Molinari F. Atherosclerosis disease management. New York: Springer-Verlag, 2010.
  20. Saba L, Sanches JM, Pedro LM, et al. Multi-modality atherosclerosis imaging and diagnosis. New York: Springer, 2014.
  21. Trivedi R, Saba L, Suri JS. 3D Imaging Technologies in Atherosclerosis. New York: Springer, 2015.
  22. Radeva P, Suri JS. Vascular and Intravascular Imaging Trends, Analysis, and Challenges, Volume 1. UK: IOP Publishing, 2019.
  23. Molinari F, Zeng G, Suri JS. Intima-media thickness: setting a standard for a completely automated method of ultrasound measurement. IEEE Trans Ultrason Ferroelectr Freq Control 2010;57:1112-24. [Crossref] [PubMed]
  24. Molinari F, Pattichis CS, Zeng G, et al. Completely automated multiresolution edge snapper—a new technique for an accurate carotid ultrasound IMT measurement: clinical validation and benchmarking on a multi-institutional database. IEEE Trans Image Process 2012;21:1211-22. [Crossref] [PubMed]
  25. Saba L, Jamthikar A, Gupta D, et al. Global perspective on carotid intima-media thickness and plaque: should the current measurement guidelines be revisited? Int Angiol 2019;38:451-65. [PubMed]
  26. Acharya UR, Krishnan MMR, Sree SV, et al. Plaque tissue characterization and classification in ultrasound carotid scans: A paradigm for vascular feature amalgamation. IEEE Trans Instrum Meas 2013;62:392-400. [Crossref]
  27. Saba L, Banchhor SK, Suri HS, et al. Accurate cloud-based smart IMT measurement, its validation and stroke risk stratification in carotid ultrasound: A web-based point-of-care tool for multicenter clinical trial. Comput Biol Med 2016;75:217-34. [Crossref] [PubMed]
  28. Saba L, Molinari F, Meiburger KM, et al. What is the correct distance measurement metric when measuring carotid ultrasound intima-media thickness automatically? Int Angiol 2012;31:483-9. [PubMed]
  29. Saba L, Meiburger KM, Molinari F, et al. Carotid IMT variability (IMTV) and its validation in symptomatic versus asymptomatic Italian population: can this be a useful index for studying symptomaticity? Echocardiography 2012;29:1111-9. [Crossref] [PubMed]
  30. Cuadrado-Godia E, Maniruzzaman M, Araki T, et al. Morphologic TPA (mTPA) and composite risk score for moderate carotid atherosclerotic plaque is strongly associated with HbA1c in diabetes cohort. Comput Biol Med 2018;101:128-45. [Crossref] [PubMed]
  31. Ikeda N, Dey N, Sharma A, et al. Automated segmental-IMT measurement in thin/thick plaque with bulb presence in carotid ultrasound from multiple scanners: Stroke risk assessment. Comput Methods Programs Biomed 2017;141:73-81. [Crossref] [PubMed]
  32. Ikeda N, Araki T, Dey N, et al. Automated and accurate carotid bulb detection, its verification and validation in low quality frozen frames and motion video. Int Angiol 2014;33:573-89. [PubMed]
  33. Molinari F, Krishnamurthi G, Acharya UR, et al. Hypothesis validation of far-wall brightness in carotid-artery ultrasound for feature-based IMT measurement using a combination of level-set segmentation and registration. IEEE Trans Instrum Meas 2012;61:1054-63. [Crossref]
  34. Saba L, Montisci R, Molinari F, et al. Comparison between manual and automated analysis for the quantification of carotid wall by using sonography. A validation study with CT. Eur J Radiol 2012;81:911-8. [Crossref] [PubMed]
  35. Saba L, Banchhor SK, Araki T, et al. Intra-and inter-operator reproducibility of automated cloud-based carotid lumen diameter ultrasound measurement. Indian Heart J 2018;70:649-64. [Crossref] [PubMed]
  36. Saba L, Jamthikar A, Gupta D, et al. Global perspective on carotid intima-media thickness and plaque: should the current measurement guidelines be revisited? Int Angiol 2019;38:451-65. [PubMed]
  37. Khanna NN, Jamthikar AD, Gupta D, et al. Performance evaluation of 10-year ultrasound image-based stroke/cardiovascular (CV) risk calculator by comparing against ten conventional CV risk calculators: A diabetic study. Comput Biol Med 2019;105:125-43. [Crossref] [PubMed]
  38. Khanna NN, Jamthikar AD, Gupta D, et al. Effect of carotid image-based phenotypes on cardiovascular risk calculator: AECRS1.0. Med Biol Eng Comput 2019;57:1553-66. [Crossref] [PubMed]
  39. Suri J, Turk M, Jamthikar A, et al. Performance evaluation of AECRS 1.0 using stroke risk calculators. European Journal of Neurology; 2019: Wiley 111 River ST, Hoboken 7030-5774, NJ USA.
  40. Khanna NN, Jamthikar AD, Araki T, et al. Nonlinear model for the carotid artery disease 10-year risk prediction by fusing conventional cardiovascular factors to carotid ultrasound image phenotypes: A Japanese diabetes cohort study. Echocardiography 2019;36:345-361. [Crossref] [PubMed]
  41. Bodai BI, Nakata TE, Wong WT, et al. Lifestyle Medicine: A Brief Review of Its Dramatic Impact on Health and Survival. Perm J 2018;22:17-025. [PubMed]
  42. Grundy SM. Age as a risk factor: you are as old as your arteries. Am J Cardiol 1999;83:1455-7, A7.
  43. Dakik HA. Vascular age for predicting cardiovascular risk: A novel clinical marker or just a mathematical permutation. J Nucl Cardiol 2019;26:1356-7. [Crossref] [PubMed]
  44. Cuende JI. Vascular age versus cardiovascular risk: Clarifying concepts. Revista Española de Cardiología 1970.243-6. [PubMed]
  45. Stein JH, Fraizer MC, Aeschlimann SE, et al. Vascular age: Integrating carotid intima‐media thickness measurements with global coronary risk assessment. Clin Cardiol 2004;27:388-92. [Crossref] [PubMed]
  46. Adolphe AB, Huang X, Cook LS. Carotid intima-media thickness determined vascular age and the framingham risk score. Crit Pathw Cardiol 2011;10:173-9. [Crossref] [PubMed]
  47. Mikael LdR, Paiva AMG, Gomes MM, et al. Vascular aging and arterial stiffness. Arq Bras Cardiol 2017;109:253-8. [PubMed]
  48. Iurciuc S, Cimpean AM, Mitu F, et al. Vascular aging and subclinical atherosclerosis: why such a "never ending" and challenging story in cardiology? Clin Interv Aging 2017;12:1339-45. [Crossref] [PubMed]
  49. Perk J, De Backer G, Gohlke H, et al. European Guidelines on cardiovascular disease prevention in clinical practice (version 2012): The Fifth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of nine societies and by invited experts). Atherosclerosis 2012;223:1-68. [Crossref] [PubMed]
  50. Gepner AD, Keevil JG, Wyman RA, et al. Use of carotid intima-media thickness and vascular age to modify cardiovascular risk prediction. J Am Soc Echocardiogr 2006;19:1170-4. [Crossref] [PubMed]
  51. Khalil Y, Mukete B, Durkin MJ, et al. A comparison of assessment of coronary calcium vs carotid intima media thickness for determination of vascular age and adjustment of the Framingham Risk Score. Prev Cardiol 2010;13:117-21. [PubMed]
  52. Cuende JI, Cuende N, Calaveras-Lagartos J. How to calculate vascular age with the SCORE project scales: a new method of cardiovascular risk evaluation. Eur Heart J 2010;31:2351-8. [Crossref] [PubMed]
  53. Romanens M, Ackermann F, Sudano I, et al. Arterial age as a substitute for chronological age in the AGLA risk function could improve coronary risk prediction. Swiss Med Wkly 2014;144:w13967. [PubMed]
  54. Saba L, Dey N, Ashour AS, et al. Automated stratification of liver disease in ultrasound: An online accurate feature classification paradigm. Comput Methods Programs Biomed 2016;130:118-34. [Crossref] [PubMed]
  55. Saba L, Jain PK, Suri HS, et al. Plaque Tissue Morphology-Based Stroke Risk Stratification Using Carotid Ultrasound: A Polling-Based PCA Learning Paradigm. J Med Syst 2017;41:98. [Crossref] [PubMed]
  56. Araki T, Ikeda N, Dey N, et al. Shape‐based approach for coronary calcium lesion volume measurement on intravascular ultrasound imaging and its association with carotid intima‐media thickness. J Ultrasound Med 2015;34:469-82. [Crossref] [PubMed]
  57. Sanches JM, Laine AF, Suri JS. Ultrasound Imaging: Advances and Applications. New York: Springer, 2012.
  58. Junyent M, Zambón D, Gilabert R, et al. Carotid atherosclerosis and vascular age in the assessment of coronary heart disease risk beyond the Framingham Risk Score. Atherosclerosis 2008;196:803-9. [Crossref] [PubMed]
  59. Naqvi TZ, Mendoza F, Rafii F, et al. High prevalence of ultrasound detected carotid atherosclerosis in subjects with low Framingham risk score: potential implications for screening for subclinical atherosclerosis. J Am Soc Echocardiogr 2010;23:809-15. [Crossref] [PubMed]
  60. de Andrade CRM Jr, Silva ELC, da Matta MFB, et al. Vascular or chronological age: which is the better marker to estimate the cardiovascular risk in patients with type 1 diabetes? Acta Diabetol 2016;53:925-33. [Crossref] [PubMed]
  61. Molinari F, Meiburger KM, Zeng G, et al. Automated carotid IMT measurement and its validation in low contrast ultrasound database of 885 patient Indian population epidemiological study: results of AtheroEdge™ Software. Int Angiol 2012;31:42-53. [PubMed]
  62. Maniruzzaman M, Jahanur Rahman M, Ahammed B, et al. Statistical characterization and classification of colon microarray gene expression data using multiple machine learning paradigms. Comput Methods Programs Biomed 2019;176:173-93. [Crossref] [PubMed]
  63. Jamthikar A, Gupta D, Khanna NN, et al. A Special Report on Changing Trends in Preventive Stroke/Cardiovascular Risk Assessment Via B-Mode Ultrasonography. Curr Atheroscler Rep 2019;21:25. [Crossref] [PubMed]
  64. Cuadrado-Godia E, Jamthikar AD, Gupta D, et al. Ranking of stroke and cardiovascular risk factors for an optimal risk calculator design: Logistic regression approach. Comput Biol Med 2019;108:182-95. [Crossref] [PubMed]
  65. Saba L, Banchhor SK, Londhe ND, et al. Web-based accurate measurements of carotid lumen diameter and stenosis severity: An ultrasound-based clinical tool for stroke risk assessment during multicenter clinical trials. Comput Biol Med 2017;91:306-17. [Crossref] [PubMed]
  66. Araki T, Ikeda N, Shukla D, et al. PCA-based polling strategy in machine learning framework for coronary artery disease risk assessment in intravascular ultrasound: A link between carotid and coronary grayscale plaque morphology. Comput Methods Programs Biomed 2016;128:137-58. [Crossref] [PubMed]
  67. Molinari F, Meiburger KM, Saba L, et al. Ultrasound IMT measurement on a multi-ethnic and multi-institutional database: our review and experience using four fully automated and one semi-automated methods. Comput Methods Programs Biomed 2012;108:946-60. [Crossref] [PubMed]
  68. Qualtrics. Determining Sample Size: How to Ensure You Get the Correct Sample Size. 2019. Available online: https://www.qualtrics.com/experience-management/research/determine-sample-size/. Accessed 4 July 2019.
  69. Sharma KH, Sahoo S, Shah KH, et al. Are Gujarati Asian Indians ‘older’ for their ‘vascular age’ as compared to their ‘Chronological age’? QJM 2015;108:105-12. [Crossref] [PubMed]
  70. Conroy R, Pyörälä K. Estimation of ten-year risk of fatal cardiovascular disease in Europe: the SCORE project. Eur Heart J 2003;24:987-1003. [Crossref] [PubMed]
  71. Srivastava SK, Singh SK, Suri JS. Effect of incremental feature enrichment on healthcare text classification system: A machine learning paradigm. Comput Methods Programs Biomed 2019;172:35-51. [Crossref] [PubMed]
  72. Acharya UR, Mookiah MR, Vinitha Sree S, et al. Atherosclerotic plaque tissue characterization in 2D ultrasound longitudinal carotid scans for automated classification: a paradigm for stroke risk assessment. Med Biol Eng Comput 2013;51:513-23. [Crossref] [PubMed]
  73. Sharma AM, Gupta A, Kumar PK, et al. A review on carotid ultrasound atherosclerotic tissue characterization and stroke risk stratification in machine learning framework. Curr Atheroscler Rep 2015;17:55. [Crossref] [PubMed]
  74. Saba L, Biswas M, Suri HS, et al. Ultrasound-based carotid stenosis measurement and risk stratification in diabetic cohort: a deep learning paradigm. Cardiovasc Diagn Ther 2019;9:439-61. [Crossref] [PubMed]
  75. Saba L, Biswas M, Kuppili V, et al. The present and future of deep learning in radiology. Eur J Radiol 2019;114:14-24. [Crossref] [PubMed]
  76. Biswas M, Kuppili V, Saba L, et al. State-of-the-art review on deep learning in medical imaging. Front Biosci (Landmark Ed) 2019;24:392-426. [Crossref] [PubMed]
  77. Biswas M, Kuppili V, Araki T, et al. Deep learning strategy for accurate carotid intima-media thickness measurement: An ultrasound study on Japanese diabetic cohort. Comput Biol Med 2018;98:100-17. [Crossref] [PubMed]
Cite this article as: Jamthikar A, Gupta D, Cuadrado-Godia E, Puvvula A, Khanna NN, Saba L, Viskovic K, Mavrogeni S, Turk M, Laird JR, Pareek G, Miner M, Sfikakis PP, Protogerou A, Kitas GD, Shankar C, Nicolaides A, Viswanathan V, Sharma A, Suri JS. Ultrasound-based stroke/cardiovascular risk stratification using Framingham Risk Score and ASCVD Risk Score based on “Integrated Vascular Age” instead of “Chronological Age”: a multi-ethnic study of Asian Indian, Caucasian, and Japanese cohorts. Cardiovasc Diagn Ther 2020;10(4):939-954. doi: 10.21037/cdt.2020.01.16